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 The mechanism of action for the antibacterial effect of tetracyclines relies on disrupting protein translation in bacteria, thereby damaging the ability of microbes to grow and repair; however protein translation is also disrupted in eukaryotic mitochondria leading to effects that may confound experimental results. The regulated gene expression system of the invention comprises a prokaryotic host organism which carries expressible nucleic acid encoding a modified tetracycline repressor of the present invention, and a target gene of which the transcription is to be regulated specifically and which is operatively linked to a promoter and at least one tet operator sequence?
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These regulations set forth a fair and rule-bound process for considering claims for protection under the Convention! Such stable cell lines can then be used to clone such toxic genes (eg, inducing the expression of the toxic genes under controlled conditions using a substituted tetracycline compound).
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